Evaluation of SARS-CoV-2 RNA shedding in clinical specimens and clinical characteristics of 10 patients with COVID-19 in Macau
Identifieur interne : 000658 ( 2020/Analysis ); précédent : 000657; suivant : 000659Evaluation of SARS-CoV-2 RNA shedding in clinical specimens and clinical characteristics of 10 patients with COVID-19 in Macau
Auteurs : Iek Long Lo [République populaire de Chine] ; Chon Fu Lio [République populaire de Chine] ; Hou Hon Cheong [République populaire de Chine] ; Chin Ion Lei [République populaire de Chine] ; Tak Hong Cheong [République populaire de Chine] ; Xu Zhong [République populaire de Chine] ; Yakun Tian [République populaire de Chine] ; Nin Ngan Sin [République populaire de Chine]Source :
- International Journal of Biological Sciences [ 1449-2288 ] ; 2020.
Descripteurs français
- KwdFr :
- ARN viral (isolement et purification), Adulte, Adulte d'âge moyen, Diarrhée (virologie), Excrétion virale, Expectoration (virologie), Femelle, Fièvre (virologie), Fèces (virologie), Humains, Infections à coronavirus (), Infections à coronavirus (diagnostic), Infections à coronavirus (traitement médicamenteux), Infections à coronavirus (virologie), Macao, Mâle, Pandémies, Partie nasale du pharynx (virologie), Pneumopathie virale (), Pneumopathie virale (diagnostic), Pneumopathie virale (virologie), Poumon (imagerie diagnostique), RT-PCR, Radiographie thoracique, Réaction de polymérisation en chaine en temps réel, Techniques de laboratoire clinique, Tomodensitométrie, Urine (virologie), Études rétrospectives.
- MESH :
- diagnostic : Infections à coronavirus, Pneumopathie virale.
- imagerie diagnostique : Poumon.
- isolement et purification : ARN viral.
- traitement médicamenteux : Infections à coronavirus.
- virologie : Diarrhée, Expectoration, Fièvre, Fèces, Infections à coronavirus, Partie nasale du pharynx, Pneumopathie virale, Urine.
- Adulte, Adulte d'âge moyen, Excrétion virale, Femelle, Humains, Infections à coronavirus, Macao, Mâle, Pandémies, Pneumopathie virale, RT-PCR, Radiographie thoracique, Réaction de polymérisation en chaine en temps réel, Techniques de laboratoire clinique, Tomodensitométrie, Études rétrospectives.
English descriptors
- KwdEn :
- Adult, Betacoronavirus, Clinical Laboratory Techniques, Coronavirus Infections (diagnosis), Coronavirus Infections (drug therapy), Coronavirus Infections (therapy), Coronavirus Infections (virology), Diarrhea (virology), Feces (virology), Female, Fever (virology), Humans, Lung (diagnostic imaging), Macau, Male, Middle Aged, Nasopharynx (virology), Pandemics, Pneumonia, Viral (diagnosis), Pneumonia, Viral (therapy), Pneumonia, Viral (virology), RNA, Viral (isolation & purification), Radiography, Thoracic, Real-Time Polymerase Chain Reaction, Retrospective Studies, Reverse Transcriptase Polymerase Chain Reaction, Sputum (virology), Tomography, X-Ray Computed, Urine (virology), Virus Shedding.
- MESH :
- chemical , isolation & purification : RNA, Viral.
- geographic : Macau.
- diagnosis : Coronavirus Infections, Pneumonia, Viral.
- diagnostic imaging : Lung.
- drug therapy : Coronavirus Infections.
- therapy : Coronavirus Infections, Pneumonia, Viral.
- virology : Coronavirus Infections, Diarrhea, Feces, Fever, Nasopharynx, Pneumonia, Viral, Sputum, Urine.
- Adult, Betacoronavirus, Clinical Laboratory Techniques, Female, Humans, Male, Middle Aged, Pandemics, Radiography, Thoracic, Real-Time Polymerase Chain Reaction, Retrospective Studies, Reverse Transcriptase Polymerase Chain Reaction, Tomography, X-Ray Computed, Virus Shedding.
Abstract
As a city famous for tourism, the public healthcare system of Macau SAR has been under great pressure during the outbreak of the Coronavirus Disease 2019 (COVID-19). In this study, we report clinical and microbiological features of ten COVID-19 patients enrolled in the Centro Hospitalar Conde de São Januário (CHCSJ) between January 21 to February 16, 2020. Clinical samples from all patients including nasopharyngeal swab (NPS)/sputum, urine, and feces were collected for serial virus RNA testing by standard qRT-PCR assay. In total, seven were imported cases and three were local cases. The median duration from Macau arrival to admission in imported cases was 3 days. Four patients required oxygen therapy but none of them needed machinal ventilation. No fatal cases were noted. The most common symptoms were fever (80%) and diarrhea (80%). In the "Severe" group, there was significantly more elderly patients (p=0.045), higher lactate dehydrogenase levels (p=0.002), and elevated C-Reactive protein levels compared to the "Mild to Moderate" group (p<0.001). There were positive SARS-CoV-2 RNA signals in all patients' NPS and stool specimens but negative in all urine specimens. Based on our data on SARS-CoV-2 RNA shedding in stool and the possibility of a lag in viral detection in NPS specimens, the assessment of both fecal and respiratory specimen is recommended to enhance diagnostic sensitivity, and also to aid discharge decision before the role of viral RNA shedding in stool is clarified.
Url:
DOI: 10.7150/ijbs.45357
PubMed: 32226287
PubMed Central: 7098032
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 001074
- to stream Pmc, to step Curation: 001074
- to stream Pmc, to step Checkpoint: 000258
- to stream PubMed, to step Corpus: 000466
- to stream PubMed, to step Curation: 000466
- to stream PubMed, to step Checkpoint: 000523
- to stream Ncbi, to step Merge: 003617
- to stream Ncbi, to step Curation: 003617
- to stream Ncbi, to step Checkpoint: 003617
- to stream Main, to step Merge: 000658
- to stream Main, to step Curation: 000658
- to stream Main, to step Exploration: 000658
- to stream 2020, to step Extraction: 000658
Links to Exploration step
PMC:7098032Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Evaluation of SARS-CoV-2 RNA shedding in clinical specimens and clinical characteristics of 10 patients with COVID-19 in Macau</title><author><name sortKey="Lo, Iek Long" sort="Lo, Iek Long" uniqKey="Lo I" first="Iek Long" last="Lo">Iek Long Lo</name><affiliation wicri:level="1"><nlm:aff id="A1">Physician, Consultant, Department of respiratory medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Physician, Consultant, Department of respiratory medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR</wicri:regionArea><wicri:noRegion>Macau SAR</wicri:noRegion></affiliation></author><author><name sortKey="Lio, Chon Fu" sort="Lio, Chon Fu" uniqKey="Lio C" first="Chon Fu" last="Lio">Chon Fu Lio</name><affiliation wicri:level="1"><nlm:aff id="A2">Physician, Macau Academy of Medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Physician, Macau Academy of Medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR</wicri:regionArea><wicri:noRegion>Macau SAR</wicri:noRegion></affiliation></author><author><name sortKey="Cheong, Hou Hon" sort="Cheong, Hou Hon" uniqKey="Cheong H" first="Hou Hon" last="Cheong">Hou Hon Cheong</name><affiliation wicri:level="1"><nlm:aff id="A3">Physician, Macau Academy of Medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Physician, Macau Academy of Medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR</wicri:regionArea><wicri:noRegion>Macau SAR</wicri:noRegion></affiliation></author><author><name sortKey="Lei, Chin Ion" sort="Lei, Chin Ion" uniqKey="Lei C" first="Chin Ion" last="Lei">Chin Ion Lei</name><affiliation wicri:level="1"><nlm:aff id="A4">Physician, Chief of service, Department of medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Physician, Chief of service, Department of medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR</wicri:regionArea><wicri:noRegion>Macau SAR</wicri:noRegion></affiliation></author><author><name sortKey="Cheong, Tak Hong" sort="Cheong, Tak Hong" uniqKey="Cheong T" first="Tak Hong" last="Cheong">Tak Hong Cheong</name><affiliation wicri:level="1"><nlm:aff id="A5">Physician, Chief of service, Department of respiratory medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Physician, Chief of service, Department of respiratory medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR</wicri:regionArea><wicri:noRegion>Macau SAR</wicri:noRegion></affiliation></author><author><name sortKey="Zhong, Xu" sort="Zhong, Xu" uniqKey="Zhong X" first="Xu" last="Zhong">Xu Zhong</name><affiliation wicri:level="1"><nlm:aff id="A6">Physician, Consultant, Department of respiratory medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Physician, Consultant, Department of respiratory medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR</wicri:regionArea><wicri:noRegion>Macau SAR</wicri:noRegion></affiliation></author><author><name sortKey="Tian, Yakun" sort="Tian, Yakun" uniqKey="Tian Y" first="Yakun" last="Tian">Yakun Tian</name><affiliation wicri:level="1"><nlm:aff id="A7">Physician, Department of infectious diseases, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Physician, Department of infectious diseases, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR</wicri:regionArea><wicri:noRegion>Macau SAR</wicri:noRegion></affiliation></author><author><name sortKey="Sin, Nin Ngan" sort="Sin, Nin Ngan" uniqKey="Sin N" first="Nin Ngan" last="Sin">Nin Ngan Sin</name><affiliation wicri:level="1"><nlm:aff id="A8">Physician, Macau Academy of Medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Physician, Macau Academy of Medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR</wicri:regionArea><wicri:noRegion>Macau SAR</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">32226287</idno><idno type="pmc">7098032</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7098032</idno><idno type="RBID">PMC:7098032</idno><idno type="doi">10.7150/ijbs.45357</idno><date when="2020">2020</date><idno type="wicri:Area/Pmc/Corpus">001074</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001074</idno><idno type="wicri:Area/Pmc/Curation">001074</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001074</idno><idno type="wicri:Area/Pmc/Checkpoint">000258</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000258</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:32226287</idno><idno type="wicri:Area/PubMed/Corpus">000466</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000466</idno><idno type="wicri:Area/PubMed/Curation">000466</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000466</idno><idno type="wicri:Area/PubMed/Checkpoint">000523</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000523</idno><idno type="wicri:Area/Ncbi/Merge">003617</idno><idno type="wicri:Area/Ncbi/Curation">003617</idno><idno type="wicri:Area/Ncbi/Checkpoint">003617</idno><idno type="wicri:Area/Main/Merge">000658</idno><idno type="wicri:Area/Main/Curation">000658</idno><idno type="wicri:Area/Main/Exploration">000658</idno><idno type="wicri:Area/2020/Extraction">000658</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Evaluation of SARS-CoV-2 RNA shedding in clinical specimens and clinical characteristics of 10 patients with COVID-19 in Macau</title><author><name sortKey="Lo, Iek Long" sort="Lo, Iek Long" uniqKey="Lo I" first="Iek Long" last="Lo">Iek Long Lo</name><affiliation wicri:level="1"><nlm:aff id="A1">Physician, Consultant, Department of respiratory medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Physician, Consultant, Department of respiratory medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR</wicri:regionArea><wicri:noRegion>Macau SAR</wicri:noRegion></affiliation></author><author><name sortKey="Lio, Chon Fu" sort="Lio, Chon Fu" uniqKey="Lio C" first="Chon Fu" last="Lio">Chon Fu Lio</name><affiliation wicri:level="1"><nlm:aff id="A2">Physician, Macau Academy of Medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Physician, Macau Academy of Medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR</wicri:regionArea><wicri:noRegion>Macau SAR</wicri:noRegion></affiliation></author><author><name sortKey="Cheong, Hou Hon" sort="Cheong, Hou Hon" uniqKey="Cheong H" first="Hou Hon" last="Cheong">Hou Hon Cheong</name><affiliation wicri:level="1"><nlm:aff id="A3">Physician, Macau Academy of Medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Physician, Macau Academy of Medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR</wicri:regionArea><wicri:noRegion>Macau SAR</wicri:noRegion></affiliation></author><author><name sortKey="Lei, Chin Ion" sort="Lei, Chin Ion" uniqKey="Lei C" first="Chin Ion" last="Lei">Chin Ion Lei</name><affiliation wicri:level="1"><nlm:aff id="A4">Physician, Chief of service, Department of medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Physician, Chief of service, Department of medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR</wicri:regionArea><wicri:noRegion>Macau SAR</wicri:noRegion></affiliation></author><author><name sortKey="Cheong, Tak Hong" sort="Cheong, Tak Hong" uniqKey="Cheong T" first="Tak Hong" last="Cheong">Tak Hong Cheong</name><affiliation wicri:level="1"><nlm:aff id="A5">Physician, Chief of service, Department of respiratory medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Physician, Chief of service, Department of respiratory medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR</wicri:regionArea><wicri:noRegion>Macau SAR</wicri:noRegion></affiliation></author><author><name sortKey="Zhong, Xu" sort="Zhong, Xu" uniqKey="Zhong X" first="Xu" last="Zhong">Xu Zhong</name><affiliation wicri:level="1"><nlm:aff id="A6">Physician, Consultant, Department of respiratory medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Physician, Consultant, Department of respiratory medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR</wicri:regionArea><wicri:noRegion>Macau SAR</wicri:noRegion></affiliation></author><author><name sortKey="Tian, Yakun" sort="Tian, Yakun" uniqKey="Tian Y" first="Yakun" last="Tian">Yakun Tian</name><affiliation wicri:level="1"><nlm:aff id="A7">Physician, Department of infectious diseases, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Physician, Department of infectious diseases, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR</wicri:regionArea><wicri:noRegion>Macau SAR</wicri:noRegion></affiliation></author><author><name sortKey="Sin, Nin Ngan" sort="Sin, Nin Ngan" uniqKey="Sin N" first="Nin Ngan" last="Sin">Nin Ngan Sin</name><affiliation wicri:level="1"><nlm:aff id="A8">Physician, Macau Academy of Medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Physician, Macau Academy of Medicine, Centro Hospitalar Conde de São Januário, Macau Health Bureau, Macau SAR</wicri:regionArea><wicri:noRegion>Macau SAR</wicri:noRegion></affiliation></author></analytic><series><title level="j">International Journal of Biological Sciences</title><idno type="eISSN">1449-2288</idno><imprint><date when="2020">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Betacoronavirus</term><term>Clinical Laboratory Techniques</term><term>Coronavirus Infections (diagnosis)</term><term>Coronavirus Infections (drug therapy)</term><term>Coronavirus Infections (therapy)</term><term>Coronavirus Infections (virology)</term><term>Diarrhea (virology)</term><term>Feces (virology)</term><term>Female</term><term>Fever (virology)</term><term>Humans</term><term>Lung (diagnostic imaging)</term><term>Macau</term><term>Male</term><term>Middle Aged</term><term>Nasopharynx (virology)</term><term>Pandemics</term><term>Pneumonia, Viral (diagnosis)</term><term>Pneumonia, Viral (therapy)</term><term>Pneumonia, Viral (virology)</term><term>RNA, Viral (isolation & purification)</term><term>Radiography, Thoracic</term><term>Real-Time Polymerase Chain Reaction</term><term>Retrospective Studies</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Sputum (virology)</term><term>Tomography, X-Ray Computed</term><term>Urine (virology)</term><term>Virus Shedding</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (isolement et purification)</term><term>Adulte</term><term>Adulte d'âge moyen</term><term>Diarrhée (virologie)</term><term>Excrétion virale</term><term>Expectoration (virologie)</term><term>Femelle</term><term>Fièvre (virologie)</term><term>Fèces (virologie)</term><term>Humains</term><term>Infections à coronavirus ()</term><term>Infections à coronavirus (diagnostic)</term><term>Infections à coronavirus (traitement médicamenteux)</term><term>Infections à coronavirus (virologie)</term><term>Macao</term><term>Mâle</term><term>Pandémies</term><term>Partie nasale du pharynx (virologie)</term><term>Pneumopathie virale ()</term><term>Pneumopathie virale (diagnostic)</term><term>Pneumopathie virale (virologie)</term><term>Poumon (imagerie diagnostique)</term><term>RT-PCR</term><term>Radiographie thoracique</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Techniques de laboratoire clinique</term><term>Tomodensitométrie</term><term>Urine (virologie)</term><term>Études rétrospectives</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Macau</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term><term>Pneumonia, Viral</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term><term>Pneumopathie virale</term></keywords><keywords scheme="MESH" qualifier="diagnostic imaging" xml:lang="en"><term>Lung</term></keywords><keywords scheme="MESH" qualifier="drug therapy" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="imagerie diagnostique" xml:lang="fr"><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="therapy" xml:lang="en"><term>Coronavirus Infections</term><term>Pneumonia, Viral</term></keywords><keywords scheme="MESH" qualifier="traitement médicamenteux" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Diarrhée</term><term>Expectoration</term><term>Fièvre</term><term>Fèces</term><term>Infections à coronavirus</term><term>Partie nasale du pharynx</term><term>Pneumopathie virale</term><term>Urine</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term><term>Diarrhea</term><term>Feces</term><term>Fever</term><term>Nasopharynx</term><term>Pneumonia, Viral</term><term>Sputum</term><term>Urine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Betacoronavirus</term><term>Clinical Laboratory Techniques</term><term>Female</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Pandemics</term><term>Radiography, Thoracic</term><term>Real-Time Polymerase Chain Reaction</term><term>Retrospective Studies</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Tomography, X-Ray Computed</term><term>Virus Shedding</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Adulte d'âge moyen</term><term>Excrétion virale</term><term>Femelle</term><term>Humains</term><term>Infections à coronavirus</term><term>Macao</term><term>Mâle</term><term>Pandémies</term><term>Pneumopathie virale</term><term>RT-PCR</term><term>Radiographie thoracique</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Techniques de laboratoire clinique</term><term>Tomodensitométrie</term><term>Études rétrospectives</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>As a city famous for tourism, the public healthcare system of Macau SAR has been under great pressure during the outbreak of the Coronavirus Disease 2019 (COVID-19). In this study, we report clinical and microbiological features of ten COVID-19 patients enrolled in the Centro Hospitalar Conde de São Januário (CHCSJ) between January 21 to February 16, 2020. Clinical samples from all patients including nasopharyngeal swab (NPS)/sputum, urine, and feces were collected for serial virus RNA testing by standard qRT-PCR assay. In total, seven were imported cases and three were local cases. The median duration from Macau arrival to admission in imported cases was 3 days. Four patients required oxygen therapy but none of them needed machinal ventilation. No fatal cases were noted. The most common symptoms were fever (80%) and diarrhea (80%). In the "Severe" group, there was significantly more elderly patients (p=0.045), higher lactate dehydrogenase levels (p=0.002), and elevated C-Reactive protein levels compared to the "Mild to Moderate" group (p<0.001). There were positive SARS-CoV-2 RNA signals in all patients' NPS and stool specimens but negative in all urine specimens. Based on our data on SARS-CoV-2 RNA shedding in stool and the possibility of a lag in viral detection in NPS specimens, the assessment of both fecal and respiratory specimen is recommended to enhance diagnostic sensitivity, and also to aid discharge decision before the role of viral RNA shedding in stool is clarified.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Zhu, N" uniqKey="Zhu N">N Zhu</name></author><author><name sortKey="Zhang, D" uniqKey="Zhang D">D Zhang</name></author><author><name sortKey="Wang, W" uniqKey="Wang W">W Wang</name></author><author><name sortKey="Li, X" uniqKey="Li X">X Li</name></author><author><name sortKey="Yang, B" uniqKey="Yang B">B Yang</name></author><author><name sortKey="Song, J" uniqKey="Song J">J Song</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Guo, L" uniqKey="Guo L">L Guo</name></author><author><name sortKey="Wei, D" uniqKey="Wei D">D Wei</name></author><author><name sortKey="Zhang, X" uniqKey="Zhang X">X Zhang</name></author><author><name sortKey="Wu, Y" uniqKey="Wu Y">Y Wu</name></author><author><name sortKey="Li, Q" uniqKey="Li Q">Q Li</name></author><author><name sortKey="Zhou, M" uniqKey="Zhou M">M Zhou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, D" uniqKey="Wang D">D Wang</name></author><author><name sortKey="Hu, B" uniqKey="Hu B">B Hu</name></author><author><name sortKey="Hu, C" uniqKey="Hu C">C Hu</name></author><author><name sortKey="Zhu, F" uniqKey="Zhu F">F Zhu</name></author><author><name sortKey="Liu, X" uniqKey="Liu X">X Liu</name></author><author><name sortKey="Zhang, J" uniqKey="Zhang J">J Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chan, Jf W" uniqKey="Chan J">JF-W Chan</name></author><author><name sortKey="Yuan, S" uniqKey="Yuan S">S Yuan</name></author><author><name sortKey="Kok, K H" uniqKey="Kok K">K-H Kok</name></author><author><name sortKey="To, Kk W" uniqKey="To K">KK-W To</name></author><author><name sortKey="Chu, H" uniqKey="Chu H">H Chu</name></author><author><name sortKey="Yang, J" uniqKey="Yang J">J Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Holshue, Ml" uniqKey="Holshue M">ML Holshue</name></author><author><name sortKey="Debolt, C" uniqKey="Debolt C">C DeBolt</name></author><author><name sortKey="Lindquist, S" uniqKey="Lindquist S">S Lindquist</name></author><author><name sortKey="Lofy, Kh" uniqKey="Lofy K">KH Lofy</name></author><author><name sortKey="Wiesman, J" uniqKey="Wiesman J">J Wiesman</name></author><author><name sortKey="Bruce, H" uniqKey="Bruce H">H Bruce</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, C" uniqKey="Huang C">C Huang</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y Wang</name></author><author><name sortKey="Li, X" uniqKey="Li X">X Li</name></author><author><name sortKey="Ren, L" uniqKey="Ren L">L Ren</name></author><author><name sortKey="Zhao, J" uniqKey="Zhao J">J Zhao</name></author><author><name sortKey="Hu, Y" uniqKey="Hu Y">Y Hu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, N" uniqKey="Chen N">N Chen</name></author><author><name sortKey="Zhou, M" uniqKey="Zhou M">M Zhou</name></author><author><name sortKey="Dong, X" uniqKey="Dong X">X Dong</name></author><author><name sortKey="Qu, J" uniqKey="Qu J">J Qu</name></author><author><name sortKey="Gong, F" uniqKey="Gong F">F Gong</name></author><author><name sortKey="Han, Y" uniqKey="Han Y">Y Han</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pan, F" uniqKey="Pan F">F Pan</name></author><author><name sortKey="Ye, T" uniqKey="Ye T">T Ye</name></author><author><name sortKey="Sun, P" uniqKey="Sun P">P Sun</name></author><author><name sortKey="Gui, S" uniqKey="Gui S">S Gui</name></author><author><name sortKey="Liang, B" uniqKey="Liang B">B Liang</name></author><author><name sortKey="Li, L" uniqKey="Li L">L Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pan, Yy" uniqKey="Pan Y">YY Pan</name></author><author><name sortKey="Guan, Hx" uniqKey="Guan H">HX Guan</name></author><author><name sortKey="Zhou, Sc" uniqKey="Zhou S">SC Zhou</name></author><author><name sortKey="Wang, Yj" uniqKey="Wang Y">YJ Wang</name></author><author><name sortKey="Li, Q" uniqKey="Li Q">Q Li</name></author><author><name sortKey="Zhu, Tt" uniqKey="Zhu T">TT Zhu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, L" uniqKey="Zhang L">L Zhang</name></author><author><name sortKey="Liu, Y" uniqKey="Liu Y">Y Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lim, J" uniqKey="Lim J">J Lim</name></author><author><name sortKey="Jeon, S" uniqKey="Jeon S">S Jeon</name></author><author><name sortKey="Shin, Hy" uniqKey="Shin H">HY Shin</name></author><author><name sortKey="Kim, Mj" uniqKey="Kim M">MJ Kim</name></author><author><name sortKey="Seong, Ym" uniqKey="Seong Y">YM Seong</name></author><author><name sortKey="Lee, Wj" uniqKey="Lee W">WJ Lee</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, G" uniqKey="Li G">G Li</name></author><author><name sortKey="De Clercq, E" uniqKey="De Clercq E">E De Clercq</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shang, L" uniqKey="Shang L">L Shang</name></author><author><name sortKey="Zhao, J" uniqKey="Zhao J">J Zhao</name></author><author><name sortKey="Hu, Y" uniqKey="Hu Y">Y Hu</name></author><author><name sortKey="Du, R" uniqKey="Du R">R Du</name></author><author><name sortKey="Cao, B" uniqKey="Cao B">B Cao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zou, L" uniqKey="Zou L">L Zou</name></author><author><name sortKey="Ruan, F" uniqKey="Ruan F">F Ruan</name></author><author><name sortKey="Huang, M" uniqKey="Huang M">M Huang</name></author><author><name sortKey="Liang, L" uniqKey="Liang L">L Liang</name></author><author><name sortKey="Huang, H" uniqKey="Huang H">H Huang</name></author><author><name sortKey="Hong, Z" uniqKey="Hong Z">Z Hong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, H" uniqKey="Zhang H">H Zhang</name></author><author><name sortKey="Kang, Z" uniqKey="Kang Z">Z Kang</name></author><author><name sortKey="Gong, H" uniqKey="Gong H">H Gong</name></author><author><name sortKey="Xu, D" uniqKey="Xu D">D Xu</name></author><author><name sortKey="Wang, J" uniqKey="Wang J">J Wang</name></author><author><name sortKey="Li, Z" uniqKey="Li Z">Z Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lan, L" uniqKey="Lan L">L Lan</name></author><author><name sortKey="Xu, D" uniqKey="Xu D">D Xu</name></author><author><name sortKey="Ye, G" uniqKey="Ye G">G Ye</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guan, Wj" uniqKey="Guan W">WJ Guan</name></author><author><name sortKey="Ni, Zy" uniqKey="Ni Z">ZY Ni</name></author><author><name sortKey="Hu, Y" uniqKey="Hu Y">Y Hu</name></author><author><name sortKey="Liang, Wh" uniqKey="Liang W">WH Liang</name></author><author><name sortKey="Ou, Cq" uniqKey="Ou C">CQ Ou</name></author><author><name sortKey="He, Jx" uniqKey="He J">JX He</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ho, As" uniqKey="Ho A">AS Ho</name></author><author><name sortKey="Sung, Jj" uniqKey="Sung J">JJ Sung</name></author><author><name sortKey="Chan Yeung, M" uniqKey="Chan Yeung M">M Chan-Yeung</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Blom, K" uniqKey="Blom K">K Blom</name></author></analytic></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Lo, Iek Long" sort="Lo, Iek Long" uniqKey="Lo I" first="Iek Long" last="Lo">Iek Long Lo</name></noRegion><name sortKey="Cheong, Hou Hon" sort="Cheong, Hou Hon" uniqKey="Cheong H" first="Hou Hon" last="Cheong">Hou Hon Cheong</name><name sortKey="Cheong, Tak Hong" sort="Cheong, Tak Hong" uniqKey="Cheong T" first="Tak Hong" last="Cheong">Tak Hong Cheong</name><name sortKey="Lei, Chin Ion" sort="Lei, Chin Ion" uniqKey="Lei C" first="Chin Ion" last="Lei">Chin Ion Lei</name><name sortKey="Lio, Chon Fu" sort="Lio, Chon Fu" uniqKey="Lio C" first="Chon Fu" last="Lio">Chon Fu Lio</name><name sortKey="Sin, Nin Ngan" sort="Sin, Nin Ngan" uniqKey="Sin N" first="Nin Ngan" last="Sin">Nin Ngan Sin</name><name sortKey="Tian, Yakun" sort="Tian, Yakun" uniqKey="Tian Y" first="Yakun" last="Tian">Yakun Tian</name><name sortKey="Zhong, Xu" sort="Zhong, Xu" uniqKey="Zhong X" first="Xu" last="Zhong">Xu Zhong</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/2020/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000658 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/2020/Analysis/biblio.hfd -nk 000658 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= 2020
|étape= Analysis
|type= RBID
|clé= PMC:7098032
|texte= Evaluation of SARS-CoV-2 RNA shedding in clinical specimens and clinical characteristics of 10 patients with COVID-19 in Macau
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/2020/Analysis/RBID.i -Sk "pubmed:32226287" \
| HfdSelect -Kh $EXPLOR_AREA/Data/2020/Analysis/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |